Constitutive activation of Stat3 signaling pathway in human colorectal carcinoma

Table 1 Clinicopathological parameters of 45 patients with colorectal carcinoma

Clinicopathological parameters		Numbers (%)
Gender	Male	24 (53.3)
	Female	21 (46.7)
Age (yr)	Range	35-81
	Mean	61.7
	Median	66.0
Primary site	Colon	25 (55.6)
	Rectum	20 (44.4)
	I	1 (0.22)
	II	24 (53.3)
	III	14 (31.1)
	IV	6 (13.3)
Depth of invasion and Lymph node involvement	T1-T2 N0	7 (15.6)
	T3-T4 N0	18 (40.0)
	T1-T2 N1-2N2	12 (26.7)
	T3-T4 N1-N2	8 (17.8)
Distant Metastasis	M0	39 (86.7)
	M1	6 (13.3)
Histological grade	G1	12 (26.7)
	G2	22 (48.9)
	G3	11 (24.4)
Tumor size	> 5 cm	22 (48.9)
	≤ 5 cm	23 (51.1)

T1: tumor invades the submucosa; T2: tumor invades the muscularis propria; T3: tumor invades through the muscularis pro-pria into the subserosa or perirectal tissues; T4: tumor directly invades other organs or structures and/or perforates visceral peritoneum; N0: no regional lymph node metastasis; N1: me-tastasis in one to three regional lymph nodes; N2: metastasis in four or more regional lymph nodes; M0: no distant metastasis; M1: distant metastasis; G1: well differentiated tumor; G2: mod-erately differentiated tumor; G3: poorly differentiated tumor.

Table 2 Expressions of p-Stat3, cyclin D1, and Bcl-x_L in colorectal carcinoma (mean ± SD)

Items	n	Percentage (%)	A Value		t	P
			Tumor	Normal
p-Stat3	45	57.8 (26/45)	114263 ± 53598	55971 ± 28762	3.573	0.002
cyclin D1	45	64.4 (29/45)	58321 ± 24872	22563 ± 11160	5.191	0.0001
Bcl-xL	45	68.9 (31/45)	71032 ± 43425	37281 ± 14622	4.627	0.0001

Table 3 Correlations between p-Stat3, cyclin D1, Bcl-x_L and clinicopathological parameters in colorectal carcinoma (mean ± SD)

Item	n	p-Stat3	P	cyclin D1	P	Bcl-xL	P
Gender
Male	24	2.7 ± 0.6	0.846	4.1 ± 1.6	0.913	3.7 ± 1.2	0.746
Female	21	2.5 ± 0.5		4.3 ± 1.8		3.3 ± 1.1
Age (yr)
≥ 65	22	2.8 ± 0.7	0.736	4.8 ± 2.1	0.825	3.4 ± 1.5	0.870
< 65	23	2.4 ± 0.4		3.6 ± 1.3		3.6 ± 0.8
Stage
III + IV	20	3.6 ± 0.6	0.026	5.2 ± 1.9	0.065	4.2 ± 1.3	0.235
I + II	25	1.8 ± 0.5		3.4 ± 1.5		3.1 ± 1.0
Histological
Grade
G1	12	2.3 ± 0.5	0.778	4.7 ± 1.8	0.732	3.9 ± 1.3	0.894
G2	22	2.4 ± 0.6	0.645	4.2 ± 1.4	0.627	3.3 ± 1.0	0.771
G3	11	3.4 ± 0.6	0.530	3.6 ± 2.2	0.565	3.6 ± 1.2	0.832
Node
Metastasis
Positive	27	3.8 ± 0.8	0.018	5.5 ± 1.9	0.041	4.4 ± 1.4	0.162
Negative	18	1.6 ± 0.4		3.3 ± 1.5		2.9 ± 1.0
Distant
Metastasis
M1	6	3.6 ± 0.8	0.638	5.4 ± 2.2	0.612	5.2 ± 1.6	0.324
M0	39	2.5 ± 0.5		4.0 ± 1.6		3.3 ± 1.1
Tumor Size
≥ 5 cm	22	3.0 ± 0.7	0.582	4.5 ± 1.8	0.776	3.8 ± 1.3	0.735
< 5 cm	23	2.2 ± 0.4		3.9 ± 1.6		3.2 ± 1.0

G1: well differentiated tumor, G2: moderately differentiated tumor, G3: poorly differentiated tumor, M0: no distant metastasis, M1: distant metastasis.