Immune tolerance in pancreatic islet xenotransplantation

Table 1 Macrophage phagocytosis in diabetic mice after pig islet xenotransplantation

Group	Pre-treatment		7 d post-treatment		14 d post-treatment
	Percent (%)	Index	Percent (%)	Index	Percent (%)	Index
Hepatocytes group	59.3 ± 4.5	1.05 ± 0.04	62.8 ± 4.37	1.28 ± 0.13	70.6 ± 3.82	1.25 ± 0.07
Splenocytes group	62.5 ± 3.0	1.04 ± 0.07	63.1 ± 6.45	1.18 ± 0.14	69.5 ± 3.62	1.21 ± 0.09
Mixture group	63.1 ± 3.8	1.07 ± 0.06	61.6 ± 3.54	1.12 ± 0.09	62.8 ± 4.70	1.09 ± 0.08
Islet group	62.0 ± 3.3	1.06 ± 0.08	73.5 ± 5.51	1.41 ± 0.18	72.7 ± 5.71	1.47 ± 0.10

Table 2 Effect of transplantation with pig islets on splenic lymphocyte transforming function in mice

Group	Pre-transplantation (cpm)	7 d post-transplantation (cpm)	14 d post-transplantation (cpm)
Hepatocytes group	11437.56 ± 1137.55	23453.74 ± 4157.28	20178.56 ± 4159.74
Splenocytes group	11437.56 ± 1137.55	25834.66 ± 3763.37	15336.25 ± 3782.38
Mixture group	11437.56 ± 1137.55	21920.05 ± 600.08	14345.53 ± 2653.28
Islets group	11437.56 ± 1137.55	32339.13 ± 5071.67	22182.16 ± 750.28

Table 3 Effect of pig islet xenotransplantation on NK activity (%) in diabetic mice

Group	Pre-treatment	7 d post-treatment	14 d post-treatment
Hepatocytes group	31.56 ± 4.23	63.17 ± 3.47	60.26 ± 7.65
Splenocytes group	31.76 ± 3.48	62.50 ± 4.86	56.19 ± 8.13
Mixture group	31.37 ± 8.09	48.00 ± 12.0	34.33 ± 14.57
Islet group	35.67 ± 3.51	76.23 ± 12.50	71.00 ± 3.0

Table 4 Variations of T lymphocytes in diabetic mice after pig islet xenotransplantation

Group	Pretreatment(%)			7 d post-treatment (%)			14 d post-treatment (%)
	CD3	CD4	CD8	CD3	CD4	CD8	CD3	CD4	CD8
Hepatocyte group	61.6 ± 4.5	42.0 ± 4.6	24.3 ± 1.5	72.7 ± 5.5	51.6 ± 4.5	28.0 ± 3.6	70.0 ± 2.0	52.7 ± 4.9	26.7 ± 4.2
Splenocyte group	66.0 ± 3.6	46.3 ± 4.0	24.6 ± 2.5	73.0 ± 4.4	53.3 ± 4.5	31.3 ± 4.1	68.7 ± 5.7	45.3 ± 4.1	33.0 ± 3.6
Mixture group	64.7 ± 2.5	44.3 ± 6.7	25.3 ± 4.9	65.0 ± 5.3	47.6 ± 3.8	28.3 ± 2.5	61.6 ± 3.5	47.0 ± 4.4	27.7 ± 2.1
Islet group	65.0 ± 7.0	43.3 ± 3.0	24.3 ± 3.2	80.0 ± 2.0	60.6 ± 3.2	32.3 ± 3.1	72.3 ± 6.7	58.7 ± 9.1	32.0 ± 4.0

Table 5 B lymphocyte antibody-forming function (A value) in mice after transplantation (A)

Group	Pretransplantation	7 d post-transplantation	14 d post-transplantation
Hepatocyte group	0.34 ± 0.06	0.44 ± 0.02	0.63 ± 0.07
Splenocyte group	0.34 ± 0.06	0.55 ± 0.02	0.63 ± 0.08
Mixture group	0.34 ± 0.06	0.55 ± 0.01	0.50 ± 0.06
Islet group	0.34 ± 0.06	0.74 ± 0.11	0.81 ± 0.13