Potential role of p53 mutation in chemical hepatocarcinogenesis of rats

Table 1 Primer sequences and PCR conditions used in sequencing DNA of p53 gene and RT-PCR

Exons	Primer	Primer sequences of p53	Conditions
5	P1	GACCTTTGATTCTTTCTCCTCTCC	94 °C , 3 min (94 °C , 30 sec; 56 °C , 30 sec; 72 °C , 30 sec) × 30
	P4	GGGAGACCCTGGACAACCAG
6/7	P2	GCCTCTGACTTATTCTTGCTC	94 °C , 3 min (94 °C , 30 sec; 56 °C , 30 sec; 72 °C , 30 sec) × 30
	P5	CCCAACCTGGCACACAGCTTC
8	P3	CTGTGCCTCCTCTTGTCCCG	94 °C , 3 min (94 °C , 30 sec; 56 °C , 30 sec; 72 °C , 30 sec) × 30
	P6	CCACCTTCTTTGTCCTGCCTG
P53	Pu	GTCGGCTCCGACTATACCACTATC	95 °C , 2 min (95 °C , 0 sec; 56 °C , 5 sec; 72 °C , 11 sec) × 30
mRNA	Pd	CTCTCTTTGCACTCCCTGGGGG
GST-P	Pu	ATCGTCCACGCAGCTTTGA	95 °C , 2 min (95 °C , 0 sec; 57 °C , 5 sec; 72 °C , 13 sec) × 30
mRNA	Pd	AGCCTCCTTCTGGTCTTTC
β-actin	Pu	ACCACCATGTACCCAGGCAT	95 °C , 2 min (95 °C , 0 sec; 56 °C , 5 sec; 72 °C , 10 sec) × 30
mRNA	Pd	CCGGACTCATCGTACTCCTG