Using yeast two-hybrid system to identify ECRG2 associated proteins and their possible interactions with ECRG2 gene

Figure 1 Colony-lift filter assay for β-galactosidase activity. Left: β-galactosidase activity of AH109 transformed with pGBKT7-ECRG2. Right: β-galactosidase activity of AH109 transformed with pCL1 (positive control). The results showed that pCL1 could activate reporter genes, but pGBKT7-ECRG2 could not.

Figure 2 Positive colonies screened by yeast two-hybrid sys-tem using full-length cDNA of ECRG2 as baits. A: PCR products of positive clones digested with HaeIII restriction enzyme. Lane 1: λDNA/EcoR I+Hind III Marker, Lanes 2-16: positive colonies, B: Trp⁺/Leu⁺/His⁺/Ade⁺ positive clone growing on the SD/Trp^-/Leu^-/His^-/Ade^- plate, C: colony-lift filter assay for β-galactosidase activity.

Figure 3 Blast results of MT2A.

Figure 4 Blast results of AF422192.