Construction and expression of a humanized M2 autoantigen trimer and its application in the diagnosis of primary biliary cirrhosis

doi:10.3748/wjg.v9.i6.1352

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Jun 15, 2003 (publication date) through Nov 23, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Clinical Research

World J Gastroenterol. Jun 15, 2003; 9(6): 1352-1355
Published online Jun 15, 2003. doi: 10.3748/wjg.v9.i6.1352

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Figure 1 Construction protocol of recombinant plasmids.

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Figure 2 Segment analysis of recombinant plasmids by restriction endonuclease digestion. 1, 7. Markers; 2. pQE-30 (Bamh1); 3. pQE-30/BCOADC-E₂ (Bamh1+Sph1); 4. pQE-30/PDC-E₂ (Sph1+Sac1); 5. p30/OGDC-E₂ (Sac1+Sal1); 6. pQE-30/BPO (Bamh1+Sal1).

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Figure 3 Expression products of recombinant plasmids detected by SDS-PAGE stained with Coomassie Brilliant Blue R-250. Lane 1: pQE-30 (control); Lane 2: pQE-30/BCOADC-E₂; Lane 3: pQE-30/PDC-E₂; Lane 4: pQE-30/OGDC-E₂; Lane 5: pQE-30/BPO; Lane 6: protein marker.

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Figure 4 Immunoreactivity of sera against recombinant proteins. Three M₂ antibody positive sera (A, B, C) and a M₂ antibody negative serum (D) were probed with SDS-PAGE-separated recombinant proteins of BCOADC-E₂ (lane a), PDC-E₂(lane b), OGDC-E₂ (lane c), BPO (lane d).

Citation: Jiang XH, Zhong RQ, Yu SQ, Hu Y, Li WW, Kong XT. Construction and expression of a humanized M₂ autoantigen trimer and its application in the diagnosis of primary biliary cirrhosis. World J Gastroenterol 2003; 9(6): 1352-1355
URL: https://www.wjgnet.com/1007-9327/full/v9/i6/1352.htm
DOI: https://dx.doi.org/10.3748/wjg.v9.i6.1352