Upregulated expression of Ezrin and invasive phenotype in malignantly transformed esophageal epithelial cells

Figure 1 RT-PCR assay of ezrin mRNA. The expression of ezrin mRNA was significantly increased in SHEEMT compared with that in SHEEIMM cells. A. Ezrin; B. GAPDH: a. Marker; b. SHEEIMM; c. SHEEMT.

Figure 2 Western blot analysis of ezrin protein expression. The ezrin protein was exhibited as a band of 81-kDa segment, whose expression was up-regulated in SHEEMT compared with that in SHEEIMM cells. Lane a: SHEEIMM; lane b: SHEEMT.

Figure 3 The inoculated SHEEMT cells (arrow) invaded into the parenchyma of pancreas (P) (HE, × 200).

Figure 4 Tumor formation in subcutaneous tissue inoculated with SHEEMT cells (C), the latter was also shown to invade and destroy nearby muscle fibers (M). (HE, × 200).

Figure 5 The invasiveness of inoculated SHEEMT cells on amniotic epithelium was demonstrated by scanning electron microscopy. A. A cluster of SHEEMT cells (T) grew on the amniotic epithelial surface (bar, 5 μ); B. On the cutting surface, SHEEMT cells (T) were observed invading into the amnion stroma (bar, 50 μ).