Immunotolerance of liver allotransplantation induced by intrathymic inoculation of donor soluble liver specific antigen

Figure 1 Rejection grades of grafted liver in different groups. Data were expressed as mean ± SD. I, Wistar-Wistar group; II, SD-Wistar group; III, SD-Wistar + CsA group; IV, LSA + SD-Wistar group; Rejection grades of group IV were significantly lower than that in group II at all but day 1 time point. No sig-nificant discrepancies were found between group IV and group III on day 7 and day 12 time points. ^aP < 0.05 vs group II, ^bP > 0.05 vs group III.

Figure 2 The kinetic change of intragraft IL-2mRNA expres-sion in different groups. I, Wistar-Wistar group; II, SD-Wistar group; III, SD-Wistar + CsA group; IV, LSA + SD-Wistar group; IL-2mRNA expression of group IV were significantly lower than that in group II at all time point (P < 0.05).

Figure 3 The kinetic change of Intragraft IFN-γ mRNA ex-pression in different groups. I, Wistar-Wistar group; II, SD-Wistar group; III, SD-Wistar + CsA group; IV, LSA + SD-Wistar group; IL-2mRNA expression of group IV were significantly lower than that in group II at all time point (P < 0.05).

Figure 4 EMSA specific competitive inhibition. Lane 1, nega-tive control; Lane 2, positive control; Lane 3, specific competition; Lane 4, non-specific competition; Lane 5, mutant competition.

Figure 5 NF-κB activity of splenocytes at different time points after the transplantation. A, Wistar-Wistar group; B, SD-Wistar group; C, SD-Wistar + CsA group; D, LSA + SD-Wistar group; E, the kinetic change of NF-κB activity. NF-κB activity of group IV were significantly lower than that in group II at all time point (P < 0. 05).