Copyright
©The Author(s) 2003.
World J Gastroenterol. Apr 15, 2003; 9(4): 714-716
Published online Apr 15, 2003. doi: 10.3748/wjg.v9.i4.714
Published online Apr 15, 2003. doi: 10.3748/wjg.v9.i4.714
Figure 1 RT-PCR product on 1% agarose gel.
Lane 1: ODC RT-PCR product, 1480 bp in length; Lane 2: pQE30-ODC plas-mid digested by restriction enzyme BamH I and Sal I.
Figure 2 The pQE30-ODC expression vector including early promoter T5, Lac operator and 6His-Tag.
Figure 3 Expressed ODC protein on SDS-PAGE.
Lane 1: M15 negative control; Lane 2-5: Expressed ODC proteins in M15 1 to 4 hour(s) after induction of IPTG; M: Protein marker; The arrow showed the expressed ODC protein.
Figure 4 Western blot shows a strip of 50 kD indicating the expression of ODC protein.
- Citation: Hu HY, Liu XX, Jiang CY, Zhang Y, Bian JF, Lu Y, Geng Z, Liu SL, Liu CH, Wang XM, Wang W. Cloning and expression of ornithine decarboxylase gene from human colorectal carcinoma. World J Gastroenterol 2003; 9(4): 714-716
- URL: https://www.wjgnet.com/1007-9327/full/v9/i4/714.htm
- DOI: https://dx.doi.org/10.3748/wjg.v9.i4.714