Construction of a regulable gene therapy vector targeting for hepatocellular carcinoma

Figure 1 Gene map of pEGFP-1-EP.

Figure 2 PCR result of gene segments. A. 200 bp DNA marker; B. enhancer of AFP; C. promoter of AFP.

Figure 3 Enzyme digestion analysis of the recombinant ex-pression vector. A. 200 bp DNA marker; B. pEGFP-1-EP (Bgl II, Sal I, BamH I); C. pEGFP-1-EP (Xho I, BamH I); D. pEGFP-1-EP (Hind III); E. pEGFP-1(EcoR I, BamH I).

Figure 4 Transient expression of EGFP. A. EGFP expressing in HepG2; B. EGFP do not express in Hela cells.

Figure 5 The expression of EGFP suppressed by ATRA. A. stable expression of EGFP in HepG2; B. expression of EGFP suppressed by 2.5 × 10^-7 M ATRA.