Different alterations of cytochrome P450 3A4 isoform and its gene expression in livers of patients with chronic liver diseases

Figure 1 Western Blot analysis of CYP3A4 isoform protein among three groups, compared with controls (tagged by “C_1-6”) . CYP3A4 protein content in liver tissues among patients with cirrhosis (tagged by “H_1-6”) reduced. but in obstructive jaundice (tagged by “O_1-6”) , There was no change of CYP3A4 protein expression.

Figure 2 The results of substratum electrophoresis in pBS M13 CYP3A4 plasmid. Lane 1: pBS M13 CYP3A4 (Hind III), Lane 2: Marker (1kb DNA ladder).

Figure 3 Northern blot analysis mRNA expression of CYP3A4 isoform in liver tissues among three groups. Total RNA was isolated without degradation and each bottom panel showed an equal amount of total RNA loading as indicated in 28 s and 18 s rRNA. CYP3A4mRNA was expressed well in human liver tissues, of patients with cirrhosis (tagged by “H”) , CYP3 A4mRNA was reduced significantly compared with control (tagged by “C”) , but no decline happened in jaundice group (tagged by “O”) .