15d-PGJ2 inhibits cell growth and induces apoptosis of MCG-803 human gastric cancer cell line

Figure 1 Effect of 15d-PGJ₂ on cell growth by H³-TdR assay (48 h). MCG-803 cells were incubated with various concentra-tions of 15d-PGJ₂ for 48 h. The value is represented as mean ± SEM (n = 3). ^aP < 0.05, ^bP < 0.01 versus control group.

Figure 2 Effect of 15d-PGJ2 on DNA fragmentation in MCG-803 cells. Cytoplasmic histone-associated DNA fragments were determined using a commercial ELISA kit. Culture of MCG-803 cells for 24 h in the presence of 15d-PGJ2 resulted in dose dependent DNA fragmentation. ^aP < 0.05, ^bP < 0.01 compared with the controls. The value is represented as mean ± SEM (n = 3).

Figure 3 COX-1, COX-2, PPARγ, bcl-2, bax, and bcl-XL pro-tein levels in MCG-803 cells treated with 15d-PGJ2. Cell ly-sates were collected and processed at 6 h. The whole cellular protein was electrophoresed in SDS-PAGE gel. Western blot was performed using antibodies against COX-1, COX-2, PPARγ, bcl-2, bax, bcl-XL. β-actin was used as a lane-loading control. (1) control, (2) 1 μM, (3) 10 μM, (4) 30 μM.

Figure 4 COX-1, COX-2, PPAR-γ, bcl-2, bax, bcl-XLmRNA levels in MCG-803 cells treated with 15d-PGJ2 for 6 h by North-ern blot. (1) control, (2) 1 μM, (3) 10 μM, (4) 30 μM.

Figure 5 Effect of 15d-PGJ₂ on PGE2 production by RIA (24 h). MCG-803 cells were incubated at various concentrations of 15d-PGJ₂ for 24 h. The value is represented as mean ± SEM (n = 3). ^aP < 0.05 and, ^bP < 0.01 versus corresponding control groups.