Viral Hepatitis
Copyright ©The Author(s) 2003.
World J Gastroenterol. Jan 15, 2003; 9(1): 112-116
Published online Jan 15, 2003. doi: 10.3748/wjg.v9.i1.112
Figure 1
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Figure 1 Detected hepatitis X protein expression in transfected Hela cells. Mr17000 X protein was detected in Hela-HBx cells. Lane1: control cells; Lane 2: Hela-HBx cells.
Figure 2
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Figure 2 Analysis of HBx gene integration in transgenic mice by PCR. 544 bp IL3 gene fragment and 346 bp fragment were amplified with positive and negative primers. Lane 1: 100 bp ladder Marker; Lane 2: C57-TgN (HBx) SMMU1 transgenic mice; Lane3: C57-TgN(HBx)SMMU3 transgenic mice; Lane 4: normal C57BL/6 mice.
Figure 3
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Figure 3 Expression of HBx gene in transgenic mice. (A) Western blot analysis of HBx gene expression in liver tissue from transgenic mouse. Mr 17000 X protein was detected with X protein mouse monoclonal antibody; Lane 1: C57-TgN(HBx) SMMU1 transgenic mice; Lane 2: normal C57BL/6 mice; (B): HBx gene expression mainly in hepatocytes cytoplasm of C57-TgN(HBx)SMMU1 transgenic mice demonstrated by immuno-histochemistry (× 132). (C, D) HBx gene Expression in hepaticyte cytoplasm of C57-TgN (HBx) SMMU1 transgenic mice detected by immunogold electron microscopy (arrows) 3C: × 20000; 3D: × 40000.