Preparation of human single chain Fv antibody against hepatitis C virus E2 protein and its identification in immunohistochemistry

Figure 1 Restriction map of HCV E2-scFv by Sfi I/Not I digestion. A, B: HCV E2-scFv; C: DNA Marker.

Figure 2 Nucleic acid and deduced amino acid sequences of scFv for HCV E2 protein GenBank accession number for this sequence is AF317001.

Figure 3 Absorbances of HCV-E2-scFv binding to E2 antigen by ELISA. (a). supernant from induced XL1-blue transformed with pCANTAB5E-E2-scFv; (b). posive control; (c). supernant from non-induced XL1-blue transformed with pCANTAB5E-E2-scFv, (d). negtive control.

Figure 4 A. Immunohistochemistry of liver tissue from health person; B. Immunohistochemistry of liver tissue from patients with chronic hepatitis C E2 antigen was detected in the cytoplasm of some liver cells.