Apoptosis of hepatoma cells SMMC-7721 induced by Ginkgo biloba seed polysaccharide

Figure 1 The UV spectrum adsorption curve of GBSP from 200 nm to 400 nm

Figure 2 The profile of Sepharose 4B gel filtration chromatography

Figure 3 Standard linear calibration curve of Sephadex G-200 chromatography

Figure 4 In the absence of GBSP treatment, the apoptosis ratio of SMMC-7721 cells was 3. 84% ± 0.55%, the cells debris was 0.46% ± 0.12% and the percentage of G2-M cells was 17.01% ± 1.28%.

Figure 5 In the presence of GBSP treatment, the apoptosis ratio of SMMC-7721 cells was 9. 13% ± 1.48%, the cells debris was 0.06% ± 0.06%, and the percentage of G2-M cells was 11.77% ± 1.50%.

Figure 6 The majority of SMMC-7721 cells were of shuttle shape without GBSP treatment (× 2500)

Figure 7 There were more spherical SMMC-7721 cells after GBSP treatment (× 2200). These cells shrunk and apoptosis bodies were formed on and around them.