Subcellular daunorubicin distribution and its relation to multidrug resistance phenotype in drug-resistant cell line SMMC-7721/R

Figure 1 Quantification of PCR. The ratio between the mdr1 or mrp and β₂M gene is expressed as described in MATERIALS AND METHODS. (a. parental cell-SMMC7721/S; b. SMMC7721/R; c. SMMC7721/R incubated with 0.5 μmol/L AS-ODN for 24 h)

Figure 2 Expression of P-gp and P₁₉₀ analyzed with Wstern blot. (a) immunoblotted with anit-P-gp antibody; (b) immunoblotted with anti-P₁₉₀ antibody. 1. parental cell-SMMC7721/S; 2. SMMC7721/R treated by AS-ODN (0.5 μmol/L, 72 h); 3. SMMC7721/R.

Figure 3 Intracellular DNR concentrations in cells treated with antisense oligonucleotids. The concentration of each AS-ODN is 0.5 mmol/L and the treatment time is 72 h. Data are the mean ± standard deviation of three independent experiments. (1. SMMC7721/R; 2. SMMC7721/S; 3. SMMC7721/R treated with AS-ODN/mdr1+mrp; 4. SMMC7721/R treated with AS-ODN/mdr1; 5. SMMC7721/R treated by AS-ODN/mrp; 6. SMMC7721/R treated with AS-ODN/nonsense.)

Figure 4 Intracellular DNR distribution in parental sensitive cells SMMC7721/S.

Figure 5 Intracellular DNR distribution in drug-resistant cells SMMC7721/R.

Figure 6 Intracellular DNR distribution in drug-resistant cells SMMC7721/R pre-treated with AS-ODN/mdr1 (0. 5 μmol/L) for 72 h.

Figure 7 Intracellular DNR distribution in drug-resistant cells SMMC7721/R treated with AS-ODN/mrp (0. 5 μmol/L) for 72 h.

Figure 8 Intracellular DNR distribution in drug-resistant cells SMMC7721/R pre-treated with AS-ODN/mdr1 and AS-ODN/mrp. Each AS-ODN concentration is 0.5 μmol/L and the treatment time is 72 h.

Figure 9 Intracellular DNR distribution in drug-resistant cells SMMC7721/R treated with nonsense oligonucleotides (0. 5 μmol/L) for 72 h.