Effect of Nimesulide on proliferation and apoptosis of human hepatoma SMMC-7721 cells

Figure 1 Inhibition rate of Nimesulide on proliferation of SMMC-7721 cells. Cells were incubated with 200 μmol/L, 300 μmol/L, 400 μmol/L Nimesulide for 2 d, 3 d, 4 d, 5 d respectively.

Figure 2 Transmission electron micrograph of SMMC-7721 cells treated with Nimesulide at the concentration of 300 μmol/L for 72 h. The picture showed early change of apoptosis, the nuclear chromatin condensation.

Figure 3 Cell apoptosis was determined by flow-cytometry, SMMC-7721 cells were treated with Nimesulide at various concentrations (0, 200, 300, 400 μmol/L respectively A to D).

Figure 4 TUNEL stain showed SMMC-7721 cells apoptosis. SMMC-7721 cells were treated with Nimesulide at various concentrations (300, 400 μmol/L A and B).