Inhibition of human telomerase in MKN-45 cell line by antisense hTR expression vector induces cell apoptosis and growth arrest

Figure 1 The expression of antisense hTR gene.

Figure 2 The result of β-Gal staining of MKN-45-lac. (× 600)

Figure 3 A: Detection of telomerase activity with the telomeric repeat amplification protocol (TRAP) assay. A 36 bp internal control band present in all samples indicated an appropriate polymerase chain reaction (PCR). B: Comparison of telomerase activity in MKN-45-ahTR and control cells. Compared with control cells, the telomerase activity in MKN-45-ahTR was inhibited by about 75%.

Figure 4 Hoechst 33258 staining of MKN-45-ahTR. (× 600) The cells undergoing apoptosis demonstrated apoptotic chromatin changes: blebbing, fragmentation and condensation under fluorescence microscope.

Figure 5 Cell growth curve.