Specific CEA-producing colorectal carcinoma cell killing with recombinant adenoviral vector containing cytosine deaminase gene

Figure 1 Schematic representation of the adenovirus shuttle plasmid, LTR, left inversed terminal repeats; pA, polyadenylation signal.

Figure 2 Map of pAdCEACD. There was a fragment of 1.8 kb containing CEA and CD after being digested with Xba I and Hind III; As there were two co_rIrestriction sites, there were three fragments after treatment with EcoR I and Hind III, 0.9 kb for LTR and CEA, 1.3 kb for CD, 2.2 kb for LTR and CEA and CD.

Figure 3 The identification of the recombinant adenovirus AdCEACD by dot blotting.

Figure 4 The identification of the recombinant adenovirus AdCEACD by PCR. a, PCR product using AdCEACD as templet; b, PCR product using pAdE1CMV as templet; c, PCR product using pAdCMVCD as templet; d, λDNA Marker.

Figure 5 Sensitivity of cells infected with AdCMVCD to 5-FC. ◆: 5-FC 0; ■: 5-FC, 15 μmol•L⁻¹; ▲: 5-FC, 150 μmol•L⁻¹.

Figure 6 Sensitivity of cells infected with AdCEACD to 5-FC. ◆: 5-FC 0; ■: 5-FC, 15 μmol•L⁻¹; ▲:5-FC, 150 μmol•L⁻¹.

Figure 7 The bystander effect of AdCEACD/5-FC system. □: without 5-FC; ■: 5-FC 150 μmol•L⁻¹