Expression and identification of recombinant soluble single-chain variable fragment of monoclonal antibody MC3

Figure 1 Detection of soluble MC3 ScFv in periplasmatic extract by Western blot. Lanes 1-4: periplasmatic extracts derived from phage clones 12, 19, 23 and 30 respectively, diluted 1:4 with PBS; Lane 5: low molecular weight protein marker.

Figure 2 Detection of soluble MC3 ScFv in TCA-precipitated extracellular fraction by Western blot. Lane 1: low molecular weight protein marker; Lane 2-5: TCA-precipitated extracellular fractions (to 1/5 of the original volume) derived from the phage clones 12, 19, 23 and 30 respectively.