Upregulation of vascular endothelial growth factor by hydrogen peroxide in human colon cancer

Math 1 Math(A1).

Figure 1 The migration of endothelial cells induced by LS174T cells was increased after the cancer cells were exposed to 10^-5 mol·L^-1 H₂O₂. The regular circles is the 8ìm pores located in PET membrane. A: induced by cancer cells without H₂O₂ treatment. B: induced by cancer cells with H₂O₂ treatment. × 200

Figure 2 Expression levels of VEGF were increased in LS174T and HCT8 after exposure to 10^-9, 10^-7 and 10^-5 mol·L^-1 H₂O₂, demonstrating a dose-de-pendent feature.

Figure 3 LS174T cells were treated with 1. 5 mg·L^-1 Act D for 4 h, followed by exposure to 10^-5 mol·L^-1 H₂O₂ for 2 4h. RT-PCR was done. The control was those without Act D treatment.

Figure 4 Effect of H₂O₂ on the half-life of VEGF mRNA in LS174T cells with or without H₂O₂ exposure following Act D treatment. No difference in half-life was showed between the two groups.

Figure 5 Measurement of nuclear and cytoplasmic NF-κB associated fluorescence by LSC. The red fluorescence represents the nuclear area, and the intensity of green fluorescence over nucleus(Fn) reflects NF-κB activity. A, B: LS174T cells without H₂O₂ treatment. C, D: Increase in NF-κB activity of LS174T cells with H₂O₂ exposure. E, F: HCT8 cells without H₂O₂ treatment. G, H: Increase in NF-κB activity of LS174T cells with H₂O₂ exposure.