Prokaryotical expression of structural and non-structural proteins of hepatitis G virus

Figure 1 Schematic representation of HGV genome and expressed proteins. A: Putative genomic organization of HGV CH strain; B: Fragments expressed from the HGV CH strain polyprotein.

Figure 2 Expression of the recombinant HGV proteins in E. coli analyzed by SDS-PAGE. A. Molecular mass standard; B.G1; C.G31; D.G6; E.G61; F.G61 in soluble form; G.G7; H.G82; I.G821; J.G61-821

Figure 3 Western blot analysis of expressed recombinant HGV proteins. A. Molecular mass standard; B.G1; C.G31; D.G6; E.G61 F.G61 in soluble form; G.G7; H.G82; I.G821; J.G61-821

Figure 4 SDS-PAGE Analysis of purified recombinant HGV proteins. Lane designations refer to purified HGV proteins or corresponding E. coli lysates: A.G1; B.purified G1; C.G31; D.purified G31; E.G61; F.purified G61; G.G821; H.purified G821; I.G61:821; J.purified G61-821

Figure 5 ELISA results of purified recombinant antigens. □ Positive sera ■ Negative sera