Brief Reports
Copyright ©The Author(s) 2001.
World J Gastroenterol. Jun 15, 2001; 7(3): 389-393
Published online Jun 15, 2001. doi: 10.3748/wjg.v7.i3.389
Figure 1
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Figure 1 Analysis of the PCR product of H. pylori ureB gene by 1.0% agarose gel electrophoresis. 1: 200 bp DNA ladder marker; 2: PCR product of ureB gene
Figure 2
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Figure 2 Identification of recombinant plasmids pPin-UreB digested with Hind III and EcoR V. 1: PCR product of ureB gene; 2: pPin-UreB plasmid digested by Hind III and EcoR V; 3: PinPointTM Xa-3 plasmid digested with Hind III and EcoR V; 4: λDNA/Hind III marker.
Figure 3
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Figure 3 Analysis of expression product of recombinant plasmid pPin-UreB in E. coli JM109 by 10% SDS-PAGE. 1, 2, 3: E. coli JM109; 4: E. coli JM109/PinPointTM Xa-3 before induction; 5: E. coli JM109/PinPointTM Xa-3 after induction with IPTG; 6, 7: E. coli JM109/pPin-UreB before induction; 8, 9; E. coli JM109/pPin-UreB after induction with IPTG; M: Molecular weight marker (212, 116, 97, 66.2, 57.5, 40) × 103.
Figure 4
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Figure 4 Analysis of recombinant fusion protein by Western-blotting. 1, 2, 3: E. coli JM109; 4: E. coli JM109/PinPointTM Xa-3 before induction; 5: E. coli JM109/PinPointTM Xa-3 after induction with IPTG; 6, 7: E. coli JM109/pPin-UreB before induction; 8, 9; E. coli JM109/pPin-UreB after induction with IPTG; M: Protein molecular weight marker (212, 116, 97, 66.2, 57.5, 40) × 103.
Figure 5
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Figure 5 Determination of the purified rUreB by 10% SDS-PAGE. 1: Protein molecular weight marker; 2: The purified rUreB