Primary porcine hepatocytes with portal vein serum cultured on microcarriers or in spheroidal aggregates

Figure 1 Hepatocytes growth curve in different culture systems on microcarriers.

Figure 2 Four hours after inoculation cell-bridge could be observed between microcarriers (under phase-contrast microscope × 200).

Figure 3 Microcarriers were arranged as a string of beads by adhered hepatocytes (under phase-contrast microscope × 400).

Figure 4 Stringed microcarriers with adhered hepatocytes (under phase contrast microscope × 730).

Figure 5 Hepatocytes looked like "cell flowers" 24 h after being cultivated in suspension and aggregated spheroids (under phase contrast microscope × 400).

Figure 6 On the d7 hepatocytes aggregated into "cell spheroids" (under phase contrast microscope × 400).

Figure 7 On the d10 hepatocytes in the forms of algoid plants (under phase contrast microscope × 200).

Figure 8 Changes of albumin synthesis in hepatocytes from different culture systems on microcarriers.

Figure 9 Changes of urea secretion in hepatocytes from different culture systems on microcarriers.

Figure 10 Dynamic changes of albumin and urea in the supernatant fluid.