Three-dimensional image of hepatocellular carcinoma under confocal laser scanning microscope

Figure 1 The transmission conventional light microscopic image was shown on the right side. The out-of-focus blur reduced the contrast and sharpness of the image. In the confocal image (left) the out-of-focus signals were cut off and only signals in focus were clearly visible × 160

Figure 2 The 10^th, 20^th, 30^th, 40^th, 50^th and 60^th plane digital images of Z-series. In the normal liver tissues of the confocal images some nuclei appeared or disappeared depending on their orientation in space. × 1000

Figure 3 Deep-focusing image showed the normal live r cells with similar round or ovoid nuclei, similar in size and homogeneous intensity of YOYO-1 iodide fluorescence as well. × 1000

Figure 4 Three-dimensional view of normal liver cells, the nuclear surface appeared smooth with homogeneous fluorescence intensity. × 1000

Figure 5 Deep-focusing image of atypical hyperplasi a liver cells, the structure of chromatin patterns inside the nucleus with homogeneous fluorescence intensity. × 1000

Figure 6 Three-dimensional view of atypical hyperplasia liver cells. × 1000

Figure 7 Deep-focusing image of HCC cells, the structure of chromatin patterns inside the nucleus with heterogeneous karyotheca thickness, irregular and coarse chromatin texture, chromatin underside the karyothecamainly. × 1000

Figure 8 Deep-focusing image of highly differentiated HCC chromatin texture. × 1000

Figure 9 Three-dimensional view of spindle cell HCC. × 1000

Figure 10 Three-dimensional view of tubular adenocarcinoma of HCC. × 1000