Expression of VEGF121 in gastric carcinoma MGC803 cell line

Figure 1 Neutralization of 5C₅ to the VEGF-induced HUVEC growth. n: no VEGF or antibody added, 0.10: VEGF 2 mg/L and antibody 5C₅ in various concentration were added.

Figure 2 RT-PCR of VEGF₁₂₁ cDNA and identific ation of recombinant PGEX2T-VEGF. 1: 585/985 bp DNA marker, 2: PCR product of VEGF from MGC803 cells, 3: PCR product of VEGF from HUVEC, 4: PGEX2T-VEGF digested with Bam-H I/Eco-R I.

Figure 3 SDS-PAGE analysis of GST-VEGF expressed in E. coli XL-1 blue-1. Standards of protei nmolecular weight, 2. Total proteins from bacterial transformed with PGEX2T-VEGF₁₂₁ without induced IPTG, 3. Proteins from bacterial induced by IPTG, 4. Protein pellet of bacterial lysis without induced IPTG, 5. Protein pellet of bacterial lysis induced by IPTG.

Figure 4 Western blot analysis of the bacterial expr essed GST-VEGF₁₂₁ by 5C₅. 1. Standards of protei nmolecular weight, 2. Uninduced bacterial protein treated with 5C₅, 3. Induced bacterial protein treated with 5C₅, 4. Induced bacterial protein treated with normal mouse IgG.