Original Articles
Copyright ©The Author(s) 1999.
World J Gastroenterol. Apr 15, 1999; 5(2): 125-127
Published online Apr 15, 1999. doi: 10.3748/wjg.v5.i2.125
Figure 1
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Figure 1 The construction of pGEM-ns3 and pMSG-ns3.
Figure 2
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Figure 2 The PCR results of pBns3. Lane 1: λ/EcoR I + Hind III marker; Lane 2, 3: negative controls; Lane 4, 5: PCR results from other primers; Lane 6: PCR products of ns3 gene annealing at 58 °C; Lane 7: PCR result from another annealing temperature.
Figure 3
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Figure 3 pGEM-ns3 digested with Nhe I and Xho I. Lane 1: λ/EcoR I + Hind III marker; Lane 2-9: colone 8-1; Lane 10: PCR marker
Figure 4
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Figure 4 pMSG-ns3 (clone 1) digested with Sac I and Hind III. Lane 1: λ/Hind I marker; Lane 2: Clone 1 digested with-Hind III; Lane 3: Clone 1 digested with Sac I; Lane 4: λ/EcoR I + Hind III marker.
Figure 5
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Figure 5 The Western-blot of the NS3 protein transf ected cells induced by DM. Lane 1: negative control (untransfected); Lane 2, 3: groups induced after by DM 24 h and 32 h (3 × 10-7 mol/L); Lane 4-6: groups induced by DM after 8 h, 24 h and 32 h (3 × 10-8 mol/L); Lane 7: uninduced group.