Preliminary study on the production of transgenic mice harboring hepatitis B virus X gene

Figure 1 Structure of recombinant plasmid PSHDX42 X: HBx gene; ↓: BamHI

Figure 2 Electrophoresis analysis of Nested PCR products amplified from DNA obtained from tail tissue. lane 1, normal mouse (negative control; lane 2 and 3 (transgenic mice) showed positive bands (280 bp). M, PCR marker (SABC).

Figure 3 Electrophoresis analysis of RT-PCR products. Lane 1, normal mouse liver tissue (negative control); lane 2 and 3 transgenic mice liver tissue showed positive bands (439 bp). M, PCR standard marker (SABC).

Figure 4 Two-month old transgenic mouse liver showed glycogen positive foci. PAS × 200