Detection of hepatoma cells in peripheral blood of HCC patients by nested RT-PCR

Figure 1 Amplification of AFP mRNA expressed in nuclear cells in peripheral blood by nested RT-PCR. Lane 1: Blood from healthy volunteers. Lanes 2-5: Titration of HepG-2 cells. Each lane includes (from left to right) 1000, 100, 10, 1 HepG-2 cells in 2 mL normal blood, respectively.

Figure 2 Amplification of AFP mRNA in HepG-2 cells by nested RT-PCR. Lane 1: Negative control. Lanes 2-9: Titration of total RNA extracted from HepG-2 cells. Each lane includes (from left to right) 5, 1, 0.5, 0.05, 0.005, 0.0005, 0.00005, 0.000005 μg total RNA, respectively.