Basic Study
Copyright ©The Author(s) 2019.
World J Gastroenterol. Dec 21, 2019; 25(47): 6813-6822
Published online Dec 21, 2019. doi: 10.3748/wjg.v25.i47.6813
Figure 1
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Figure 1 Non-SMC condensin I complex subunit D2 and non-SMC condensin II complex subunit D3 expression in intestinal tissues. A: Non-SMC condensin I complex subunit D2 expression by in situ hybridization assay (× 100, × 400); B: Non-SMC condensin II complex subunit D3 expression by in situ hybridization assay (× 100, × 400). UC: Ulcerative colitis; NCAPD2: Non-SMC condensin I complex subunit D2; NCAPD3: Non-SMC condensin II complex subunit D3.
Figure 2
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Figure 2 Inflammatory cytokines of different groups. bP < 0.01, vs NC; cP < 0.05, dP < 0.01, eP < 0.001, vs Model. NC: Normal control group; Model: Ulcerative colitis (UC) model group; si-Ctrl: Transfected with empty vector based on the UC model; si- NCAPD2: Transfected with si-NCAPD2 based on the UC model; si-NCAPD3: Transfected with si-NCAPD3 based on the UC model; si-NCAPD2+si-NCAPD3: Transfected with si-NCAPD2 and si-NCAPD3 based on the UC model; NCAPD2: Non-SMC condensin I complex subunit D2; NCAPD3: Non-SMC condensin II complex subunit D3.
Figure 3
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Figure 3 The relative protein expression levels by Western blot assay. bP < 0.001 vs NC; cP < 0.01, dP < 0.001 vs Model. NC: Normal control group; Model: Ulcerative colitis (UC) model group; si-Ctrl: Transfected with empty vector based on the UC model; si-NCAPD2: Transfected with si-NCAPD2 based on the UC model; si-NCAPD3: Transfected with si-NCAPD3 based on the UC model; si-NCAPD2 + si-NCAPD3: Transfected with si-NCAPD2 and si-NCAPD3 based on the UC model; NCAPD2: Non-SMC condensin I complex subunit D2; NCAPD3: Non-SMC condensin II complex subunit D3.
Figure 4
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Figure 4 IKK and NF-κB protein expression by immunofluorescence (× 200). NC: Normal Control group; Model: Ulcerative colitis (UC) model group; si-Ctrl: Transfected with empty vector based on the UC model; si-NCAPD2: Transfected with si-NCAPD2 based on the UC model; si-NCAPD3: Transfected with si-NCAPD3 based on the UC model; si-NCAPD2+si-NCAPD3: Transfected with si-NCAPD2 and si-NCAPD3 based on the UC model; NCAPD2: Non-SMC condensin I complex subunit D2; NCAPD3: Non-SMC condensin II complex subunit D3.