Basic Study
Copyright ©The Author(s) 2019.
World J Gastroenterol. Jun 14, 2019; 25(22): 2752-2762
Published online Jun 14, 2019. doi: 10.3748/wjg.v25.i22.2752
Figure 1
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Figure 1 Green fluorescent protein was detected 72 h after lentiviral transfection (fluorescence microscopy × 200). A: HepG2 cells in bright vision; B: HepG2 cells in green fluorescence vision.
Figure 2
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Figure 2 LV-hsa-mir-34a was transfected into HepG2 cells for 72 h. Expression of miR-34a-5p was detected by reverse transcription-polymerase chain reaction before and after transfection.
Figure 3
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Figure 3 Growth inhibition rate of HepG2 cells treated with LV-hsa-mir-34a transfection combined with doxorubicin. Dox: Doxorubicin.
Figure 4
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Figure 4 Proportion of G1 phase cells in each group after HepG2 cells were treated for 72 h. A: Blank control group; B: LV-hsa-mir-34a group; C: Doxorubicin treatment group; D: Empty vector + doxorubicin treatment group; E: LV-hsa-mir-34a + doxorubicin treatment group.
Figure 5
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Figure 5 The proportion of apoptotic cells in each group after HepG2 cells were treated for 72 h. A: Blank control group; B: LV-hsa-mir-34a group; C: Doxorubicin treatment group; D: Empty vector + doxorubicin treatment group; E: LV-hsa-mir-34a + doxorubicin treatment group.
Figure 6
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Figure 6 Effects of LV-hsa-mir-34a transfection combined with doxorubicin incubation for 72 h on the expression of related proteins in HepG2 cells. A: Blank control group; B: LV-hsa-mir-34a group; C: Doxorubicin treatment group; D: Empty vector + doxorubicin treatment group; E: LV-hsa-mir-34a + doxorubicin treatment group. MDR: Multidrug resistance protein; CDK: Cyclin-dependent kinase; SIRT: Sirtuin.