Promotion of Sema4D expression by tumor-associated macrophages: Significance in gastric carcinoma

Figure 1 CD68 and Sema4D expression in gastric carcinoma (streptavidin-peroxidase, × 400). A: Positive staining for CD68 in gastric carcinoma tissues; B: Positive staining for Sema4D in gastric carcinoma tissues.

Figure 2 Immunofluorescence staining of cells (400 ×). A: CD68-negative cells; B: CD68-positive cells.

Figure 3 Morphological changes of gastric carcinoma SGC-7901 cells. A: Control; B: Co-culture group (400 ×).

Figure 4 Tumor-associated macrophages promote SGC-7901 cell migration (200 ×, P < 0. 01).

Figure 5 Tumor-associated macrophages promote SGC-7901 cell invasion (200 ×, P < 0. 01).

Figure 6 Expression of Sema4D protein in SGC-7901 cell supernatants of the control and co-culture experimental groups.