Basic Study
Copyright ©The Author(s) 2016.
World J Gastroenterol. Feb 14, 2016; 22(6): 2071-2080
Published online Feb 14, 2016. doi: 10.3748/wjg.v22.i6.2071
Figure 1
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Figure 1 Hydrodynamic co-transfection of NICD and IDH1R132C does not lead to liver tumor formation in mice. A: Macroscopic appearance of the liver from an NICD/IDH1R132C injected mouse harvested at 22 wk post injection; B: Hematoxylin-eosin (HE) staining image of representative NICD/IDH1R132C liver tissue. Inset: Expanded view of the HE image.
Figure 2
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Figure 2 Hydrodynamic co-transfection of NICD and shP53 cannot promote liver tumor development in mice. A: Macroscopic appearance of the liver from a NICD/shP53 injected mouse harvested at 24 wk post injection; B: Hematoxylin-eosin (HE) stained image of representative NICD/shP53 liver tissue. Inset: Expanded view of the HE image.
Figure 3
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Figure 3 Hydrodynamic co-transfection of shP53 and IDH1R132C cannot lead to liver tumor in mice. A: Macroscopic appearance of the liver from a NICD/shP53 injected mouse harvested at 20 wk post injection; B: Hematoxylin-eosin (HE) stained image of representative shP53/IDH1R132C liver tissue. Inset: Expanded view of the HE image.
Figure 4
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Figure 4 NICD/shP53/IDH1R132C induced ICC formation in mice. A: Gross images of NICD/shP53/IDH1R132C injected mice harvested at 12 wk post injection (a) and 18 wk post injection (b). The arrow indicates a cystic lesion on the liver surface; B: Hematoxylin-eosin stained images of the corresponding liver sections showing liver tumors from NICD/shP53/IDH1R132C injected mice.
Figure 5
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Figure 5 Molecular characterization of NICD/shP53/IDH1R132C induced ICCs. A: Representative hematoxylin-eosin (HE) stained image of NICD/shP53/IDH1R132C ICC tumors; B: Myc tag; C: Flag tag; D: CK19; E: Ki67; F: p-AKT and G: p-ERK1/2 immunostaining in NICD/shP53/IDH1R132C ICC tumor samples; H: Picro-Sirius red staining image of a liver section showing profound desmoplastic reaction in a NICD/shP53/IDH1R132C ICC tumor sample. Inset: Expanded view of the immunostaining images.