Analysis of tumor-infiltrating gamma delta T cells in rectal cancer

Figure 1 Percentage of infiltrating γδT cells in 20 rectal cancer patients. Cells were stained with an anti-γδ TCR mAb, anti-Vδ1 mAb or anti-Vδ2 mAb and analyzed by flow cytometry. The left panels show representative histogram results from flow cytometry for γδ T cells (A), Vδ1 T cells (B), and Vδ2 T cells (C). The right panels show bar graphs of the percentage of positively stained cells from the patients.

Figure 2 Percentage of tumor-infiltrating Vδ1 and Vδ2 T cells correlated with disease T stage. A: Tumor-infiltrating Vδ1 T cells positively correlated with disease T stage; B: Tumor-infiltrating Vδ2 T cells negatively correlated with disease T stage.

Figure 3 Percentage of Vδ1 and Vδ2 T cells from cancer tissues or para-carcinoma tissues after expansion. Cells were stained with anti-CD3 mAb, anti-Vδ1 mAb or anti-Vδ2 mAb and analyzed by flow cytometry. The left panels show representative dot plots from flow cytometry for Vδ1 and Vδ2 T cells from para-carcinoma tissues (A) and rectal cancer tissues (B). The right panels show bar graphs of the percentage of positively stained cells from 20 rectal cancer patients.

Figure 4 Functional analysis of Vδ1 and Vδ2 T cells from cancer tissues or para-carcinoma tissues. A: Fresh naïve CD4 T cells were labeled with CFSE and co-cultured with Vδ1 T cells from cancer tissues or para-carcinoma tissues; B: Lysis of HR8348 cells by Vδ2 T cells at effector to target cell ratios (E:T) of 10:1, 20:1 and 40:1.