Potential roles of EZH2, Bmi-1 and miR-203 in cell proliferation and invasion in hepatocellular carcinoma cell line Hep3B

Figure 1 Quantitative real-time polymerase chain reaction detection of mRNA levels of Bmi-1, EZH2, and miR-203. The mRNA levels of Bmi-1 (A), EZH2 (B), and miR-203 (C) in HCC tissues.

Figure 2 Western blot analysis of Bmi-1 and EZH2 protein levels. A: Expression levels of Bmi-1 and EZH2 in HCC tissues and in normal tissues; B: Expression levels of Bmi-1 and EZH2 in Hep3B cells transfected with EZH2-shRNA or control vector. Lane 1 stands for the protein levels in Hep3B cells, and lane 2 stands for the protein levels in Hep3B cells transfected with EZH2-shRNA; C: Expression levels of Bmi-1 and EZH2 in Hep3B cells transfected with miR-203-shRNA or control vector. Lane 1 stands for the protein levels in Hep3B cells, and lane 2 stands for the protein levels in Hep3B cells with miR-203 overexpression.

Figure 3 Methyl thiazolyl tetrazolium analysis of the effect of EZH2 and miR-203 on the proliferation of Hep3B cells. A: Proliferation of Hep3B cells transfected with control shRNA vector or EZH2-shRNA vector; B: Proliferation of Hep3B cells transfected with control shRNA vector or miR-203-shRNA vector.

Figure 4 Flow cytometry analysis of apoptosis of Hep3B cells. A: Hep3B cells transfected with control shRNA vector; B: Hep3B cells transfected with EZH2-shRNA vector; C: Hep3B cells transfected with control shRNA vector; D: Hep3B cells transfected with miR-203-shRNA vector.

Figure 5 Analysis of Hep3B cell invasion in vitro. A: Invasion of Hep3B cells transfected with control shRNA vector; B: Invasion of Hep3B cells transfected with EZH2-shRNA; C: Invasion of Hep3B cells transfected with control shRNA vector; D: Invasion of Hep3B cells transfected with miR-203-shRNA.