Tong Xie Yao Fang relieves irritable bowel syndrome in rats via mechanisms involving regulation of 5-hydroxytryptamine and substance P

Figure 1 Flow chart of the study design and experiment procedure. IBS: Irritable bowel syndrome; TXYF: Tong Xie Yao Fang; RT-PCR: Reverse transcription-polymerase chain reaction.

Figure 2 Tong Xie Yao Fang affects 5-hydroxytryptamine and substance P protein expression in colon (A) and hypothalamus (B) tissues in each group, as revealed by immunohistochemistry staining (× 400; inserted panel, × 1600); C: Quantitative measurements with Integral Optical Density data from A and B (n = 10 for each group). a: Normal group; b: IBS model group; c: IBS model + positive group; d: IBS model + TXYF group. IBS: Irritable bowel syndrome; TXYF: Tong Xie Yao Fang.

Figure 3 Quantitative data of 5-hydroxytryptamine (A) and substance P (B) mRNA expression via reverse transcription-polymerase chain reaction detection in rat colon of the indicated groups.

Figure 4 5-hydroxytryptamine and substance P protein expression in rat colon tissues of the indicated groups. A: Representative Western blot image; B, C: Quantitative data from collected Western blot image. IBS: Irritable bowel syndrome; TXYF: Tong Xie Yao Fang.