Failure of interferon-γ pre-treated mesenchymal stem cell treatment in a patient with Crohn’s disease

Figure 1 Increase in methylprednisolone IC50 coincided with worsening symptoms. Concentration of methylprednisolone that inhibited 50% of the proliferation (IC₅₀) of peripheral mononuclear blood cells obtained from the patient at various experimental times, before and after mesenchymal stem cells infusion (arrows). Inhibition of proliferation was determined by labelling metabolically active cells (stimulated with concanavalin-A) with [methyl-³H]thymidine [12].

Figure 2 Time course of CCR7, CCR4 and CXCR4 expression by T-lymphocytes. Analysis of CCR7, CCR4 and CXCR4 expression by flow cytometry showed a transient reduction after 2 d. Expression levels were measured as mean fluorescence intensity index.