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©2014 Baishideng Publishing Group Inc.
World J Gastroenterol. Jun 21, 2014; 20(23): 7452-7460
Published online Jun 21, 2014. doi: 10.3748/wjg.v20.i23.7452
Published online Jun 21, 2014. doi: 10.3748/wjg.v20.i23.7452
Figure 1 Comparison of histological findings between the groups that underwent bile duct ligation (BDL) with/without rapamycin treatment and the control.
A: Hematoxylin and eosin (HE) staining. The bile duct ligation (BDL)+/Rapa++ group showed the smallest expansion of portal areas, with the least amount of ductular proliferation and the most mild inflammatory reaction among the BDL groups; B: Masson-Trichrome staining. The BDL+/Rapa++ group showed the lowest degree of fibrotic septa formation and deposition of collagen among BDL groups (blue); C: α-smooth muscle actin (α-SMA) staining (dark brown). The BDL+/Rapa++ group showed the lowest amount of staining among BDL groups; D: Cytokeratin 19 (CK 19) protein expression (dark brown). The BDL+/Rapa++ group showed the lowest amount of staining among BDL groups. Rapa: Rapamycin; Rapa+: Rat administered rapamycin for 28 d from the 14th day post-BDL; Rapa++: Rat administered rapamycin for 28 d beginning the first day post-BDL. Original magnification: × 100(a-d).
Figure 2 Transforming growth factor β1 and platelet-derived growth factor mRNA expression was assessed by polymerase chain reaction analysis.
A: Transforming growth factor β1 (TGFβ1); B: Platelet-derived growth factor (PDGF). BDL: Bile duct ligation; Rapa: Rapamycin; Rapa+: Rat administered rapamycin for 28 d starting the 14th day after BDL; Rapa++: Rat administered rapamycin treatment for 28 d starting the 1st day after BDL; GAPDH: Glyceraldehyde 3-phosphate dehydrogenase. aP < 0.05 vs other group; cP < 0.05 vs BDL+/Rapa++ group.
Figure 3 Western blot analysies showed the p70s6k and p-p70s6k expression levels in the advanced liver fibrosis samples.
A: 70-kDa ribosomal S6 kinase (p70s6k); B: p-p70s6k. BDL: Bile duct ligation; Rapa: Rapamycin; Rapa+: Rat administered rapamycin for 28 d beginning the 14th day post-BDL; Rapa++: Rat administered rapamycin for 28 d beginning the first day post-BDL. aP < 0.05 vs other group; cP < 0.05 vs BDL+/Rapa+ group; eP < 0.05 vs BDL+/Rapa- group.
- Citation: Kim YJ, Lee ES, Kim SH, Lee HY, Noh SM, Kang DY, Lee BS. Inhibitory effects of rapamycin on the different stages of hepatic fibrosis. World J Gastroenterol 2014; 20(23): 7452-7460
- URL: https://www.wjgnet.com/1007-9327/full/v20/i23/7452.htm
- DOI: https://dx.doi.org/10.3748/wjg.v20.i23.7452