Novel CDH1 germline mutations identified in Chinese gastric cancer patients

Figure 1 Novel variations detected in this study. A: c.44_46del TGC; B: c.-49G>T; C: c.604G>A (V202I); D: c.1320+7A>G.

Figure 2 Luciferase reporter assay for the CDH1 c. -49 G>T variant. The average relative luciferase activity (with standard deviation) is shown. The activity of CDH1 wild type was defined as 100%.

Figure 3 Results of reverse transcription polymerase chain reaction for CDH1 in patient G26 (c. 1320+7A>G, intron 9) and patient G181 [c.1224G>A(A408A), exon 9] using primers in exons 7 and 10. Both patients had an expected band of 567 bp. M: Molecular size markers; T: Tumor tissue; N: Paired normal tissue.