Increased tumor necrosis factor receptor 1 expression in human colorectal adenomas

Figure 1 Receiver operating characteristic curves for soluble tumor necrosis factor receptor-1 and 2 in patients with adenomas. ROC: Receiver operating characteristic.

Figure 2 Immunohistochemical analyses in the normal colorectal mucosa and adenoma tissues. A: Tumor necrosis factor (TNF)-α expression in the normal colorectal mucosa; B: TNF-α expression in the adenoma tissues; C: The percentage of TNF-α-positive cells; D: Tumor necrosis factor-receptor 1 (TNF-R1) expression in the normal colorectal mucosa; E: TNF-R1 expression in the adenoma tissues; F: The percentage of TNF-R1-positive cells; G: TNF-R2 expression in the normal colorectal mucosa; H: TNF-R2 expression in the adenoma tissues; I: The percentage of TNF-R2-positive cells. Box plots display median values and interquartile ranges (C, F, I). The non-outlier range is also shown. ^aP < 0.05 between NM and AD in stromal of TNF-α; ^cP < 0.05 between NM and AD in epithelial of TNF-R1. NS: Non-significant; NM: Normal mucosa; AD: Adenoma.

Figure 3 Immunohistochemical analyses in the normal colorectal mucosa and adenoma tissues. A: Phospho-c-Jun N-terminal kinase (p-JNK) expression in the normal colorectal mucosa; B: p-JNK expression in the adenoma tissues; C: The percentage of p-JNK positive cells; D: Nuclear factor-κ B (NF-κB) expression in the normal colorectal mucosa; E: NF-κB expression in the adenoma tissues; F: The percentage of NF-κB-positive cells; G: Caspase-3 expression in the normal colorectal mucosa; H: Caspase-3 expression in the adenoma tissues; I: The percentage of caspase-3-positive cells. Box plots display median values and interquartile ranges (C, F, I). The non-outlier range is also shown. ^aP < 0.05 between NM and AD in stromal of p-JNK; ^cP < 0.05 between NM and AD in epithelial of p-JNK. NS: Non-significant; NM: Normal mucosa; AD: Adenoma.

Figure 4 Confocal laser microscopic analyses of colorectal adenoma sections. A: Tumor necrosis factor receptor 1 (TNF-R1) expression; B: Phospho-c-Jun N-terminal kinase (p-JNK) expression; C: Merged image of A and B. Co-localized expression of TNF-R1 and p-JNK was noted predominantly in the epithelial cells of adenomas. Merged images showing co-localization; D: TNF-R2 expression; E: p-JNK expression; F: Merged image of D and E. Expression of TNF-R2 was localized predominantly to the stromal area of the adenoma. Merged images showed a lack of co-localization.