Comparative quantification of human intestinal bacteria based on cPCR and LDR/LCR

Figure 2 Distributions of ratio between each two bacteria. Enterobacterium was used to compare with other four species, respectively. The abscissa is the ratio of template concentration, divided into a 10-fold section. The ordinate is the frequency of each ratio section between two species. Enbac: Enterobacterium; Eubac: Eubacterium; Bifido: Bifidobacterium; L. casei: Lactobacillus casei; L. acido: Lactobacillus acidophilus.

Figure 1 Linear relationship between the initial template concentrations and fluorescence signal intensity of ligase detection reaction/ligase chain reaction in standard curve test. The results of Enterobacterium (Enbac)/Eubacterium (Eubac), Enbac/Bifidobacterium (Bifido) and Eubac/Bifido tested by ligase detection reaction probes were presented. The linearity analyses were done twice, and results had a high reproducibility (data not shown).