Probiotic Lactobacillus rhamnosus downregulates FCER1 and HRH4 expression in human mast cells

Figure 1 Schematic representation of statistically significant changes (P < 0. 05) in mast-cell gene expression after 24 h stimulation with Lactobacillus rhamnosus Lc705, Lactobacillus rhamnosus GG and the combination of four probiotic bacteria. LGG: Lactobacillus rhamnosus GG; Lc705: Lactobacillus rhamnosus Lc705.

Figure 2 Two-way hierarchical clustering of representative genes selected from the functional category analysis (Table 1). Data are average expression differences between unstimulated and bacteria-stimulated mast cells from three independent experiments at the 24-h time point. Analysis was performed using MeV software. PJS: Propionibacterium freudenreichii ssp. shermanii JS; Bb12: Bifidobacterium animalis ssp. lactis Bb12; Cpn: Chlamydia pneumoniae isolate Kajaani 6; Lc705: Lactobacillus rhamnosus Lc705; LGG: Lactobacillus rhamnosus GG; TLR1: Toll-Like receptor 1; NOD2: Nucleotide-binding oligomerization domain-containing protein 2; IL-1B: Interleukin-1β; IL-8: Interleukin-8; TNF: Tumor necrosis factor; IL-10: Interleukin-10; CDKN1B: Cyclin-dependent kinase inhibitor 1B; TLR6: Toll-Like receptor 6; CASP3: Caspase 3; CASP8: Caspase 8; CCL2: Chemokine (C-C motif) 2; CD8A: Cluster of differentiation 8 A (T cell surface glycoprotein); LCP2: Lymphocyte cytosolic protein 2; CCNA2: Cyclin A2; MAPK12: Mitogen-activated protein kinase 12; FCER1A: Fc fragment of IgE high affinity I receptor for α polypeptide; FCER1G: Fc fragment of IgE high affinity I receptor for γ polypeptide; LPGAT1: Lysophosphatidylglycerol acyltransferase 1.

Figure 3 Verification of mast-cell microarray results by quantitative reverse transcriptase-polymerase chain reaction with seven selected genes that are involved in mast-cell immune system regulation (A) and mast-cell activation (B). Gene expression was quantified after 24 h stimulation with four probiotic bacteria; Lactobacillus rhamnosus GG (LGG), Lactobacillus rhamnosus Lc705 (Lc705), Propionibacterium freudenreichii ssp. shermanii JS (PJS), Bifidobacterium animalis ssp. lactis Bb12 (Bb12) and their combination or with Chlamydia pneumoniae isolate Kajaani 6 (Cpn). Data are mean values ± SE of three independent experiments. IL: Interleukin; TNF-α: Tumor necrosis factor-α; CCL2: Chemokine (C-C motif) 2; FCER1A: Fc fragment of IgE high affinity I receptor for α polypeptide; FCER1G: Fc fragment of IgE high affinity I receptor for γ polypeptide; HRH4: Histamine H4 receptor.