Human papillomavirus DNA and P16INK4A expression in concurrent esophageal and gastric cardia cancers

Figure 1 Hematoxylin and eosin staining in the concurrent esophageal and gastric cardia cancers tissue specimens. A: Esophageal squamous cell carcinoma tissue (× 100); B: Gastric cardia adenocarcinoma tissue (× 100).

Figure 2 Amplification of human papillomavirus type 16-E6 gene fragment in the concurrent esophageal and gastric cardia cancers tissues. Polymerase chain reaction products were run in 3.0% agarose gel; Lane M: Molecular marker (100 bp ladder); Lane 1: Double water (negative control); Lane 2: Plasmid with human papillomavirus type 16 (HPV16)-E6 120 bp (positive control); Lanes 3-16: Represent positive and negative cases. A: HPV16-E6 gene fragment amplification in esophageal squamous cell carcinoma tissues; B: HPV16-E6 gene fragment in the corresponding gastric cardia adenocarcinoma tissue amplification.

Figure 3 P16^INK4A protein expression in the concurrent esophageal and gastric cardia cancers tissues by immunohistochemical staining Avidin-Biotin-Peroxidase Complex method (× 200). A: P16^INK4A protein expression in esophageal squamous cell carcinoma; B: Expression of P16^INK4A protein in gastric cardia adenocarcinoma.