Brief Article
Copyright ©2009 The WJG Press and Baishideng.
World J Gastroenterol. Dec 28, 2009; 15(48): 6117-6122
Published online Dec 28, 2009. doi: 10.3748/wjg.15.6117
Figure 1
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Figure 1 Analysis of osteoglycin expression. RT-PCR analysis (A) and Western blot analysis (B) of osteoglycin expression in mouse hepatocarcinoma cells; relative signal intensities of osteoglycin mRNA (C) and protein (D) levels were normal as against those of β-actin by LabWorks (UVP GDS-800 Version 4.0) analysis (compared with F cells, aP < 0.05). F: Hca-F cells; P: Hca-P cells; F(+): Hca-F cells transfected with pIRESpuro3 osteoglycin(+); F0: Hca-F cells transfected with pIRESpuro3. β-actin was used as an internal control.
Figure 2
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Figure 2 Metastatic lymph nodes of tumor-burden mice inoculated with Hca-F cells (A), Hca-F cells transfected with pIRESpuro3 (B), or Hca-F cells transfected with pIRESpuro3 osteoglycin(+) (C). Lymph nodes of tumor-burden mice were HE stained and examined under microscope.
Figure 3
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Figure 3 Zymographic analysis of MMPs activity of tumor cells in DMEM with or without lymph node extract (A); the intensity of gelatin lysis bands obtained by scanning densitometry (LabWorks UVP GDS-800 Version 4. 0, multiple comparisons, aP < 0.05) (B). 1. L; 2. F; 3. P; 4. F(+); 5. F0; 6. F; 7. P; 8. F(+); 9. F0. 10. Type IV collagenases. 1. L: lymph node extract; 2-5: cells in DMEM. 6-10: cells cultured with lymph node extract. F: Hca-F cells; P: Hca-P cells; F(+): Hca-F cells transfected with pIRESpuro3 osteoglycin(+); F0: Hca-F cells transfected with pIRESpuro3.
Figure 4
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Figure 4 Zymographic analysis of MMPs activity of tumor cells in liver extract (A); the intensity of gelatin lysis bands obtained by scanning densitometry (LabWorks UVP GDS-800 Version 4. 0) (B). 1. Type IV collagenases; 2. F; 3. P; 4. F(+); 5. F0; 6. Liver extract. F: Hca-F cells; P: Hca-P cells; F(+): Hca-F cells transfected with pIRESpuro3 osteoglycin(+); F0: Hca-F cells transfected with pIRESpuro3.
Figure 5
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Figure 5 Zymographic analysis of MMPs activity of tumor cells in spleen extract (A); the intensity of gelatin lysis bands obtained by scanning densitometry (LabWorks UVP GDS-800 Version 4. 0) (B). 1. Type IV collagenases; 2. F; 3. P; 4. F(+); 5. F0; 6. spleen extract. F: Hca-F cells; P: Hca-P cells; F(+): Hca-F cells transfected with pIRESpuro3 osteoglycin(+); F0: Hca-F cells transfected with pIRESpuro3.