EGFR and HER2 expression in advanced biliary tract cancer

Figure 1 Immunohistochemistry of biliary tract cancer specimens for expression of HER2 (A, C, E and G) and EGFR (B, D, F and H). A: HER2 neg.; B: EGFR neg.; C: HER2 1+; D: EGFR 1+; E: HER2 2+; F: EGFR 2+; G: HER2 3+; H: EGFR 3+.

Figure 2 Dual color FISH analysis of HER2 in cholangiocarcinoma specimens. A: Nonamplified tumor with single HER2 gene copy status. The red probe is specific for HER2. The green probe is specific for chromosome 17 centromere; B: Tumor with high amplification of the HER2 gene. There are multiple red HER2 specific signals in this tumor in relation to the green chromosome 17 signals.