Overexpression of β3/γ2 chains of laminin-5 and MMP7 in biliary cancer

Figure 1 RT-PCR analysis of the LNα3, LNβ3, LNγ2 genes in biliary tract cancer cell lines. 1: MEC; 2: TFK-1; 3: HuH28; 4: TGBC1TKB; 5: TGBC2TKB; 6: TGBC14TKB; 7: TKKK.

Figure 2 LNγ2 expression in biliary tract cancer tissues. A, B: Extrahepatic bile duct cancer tissues; A: Moderately differentiated tubular adenocarcinoma positive for staining. Note that LNγ2 is strongly positive in tumor cells at the invasive front; B: Papillary adenocarcinoma negative for staining; C, D: Gallbladder cancer tissues; C: Moderately differentiated tubular adenocarcinoma positive for staining; D: Well differentiated tubular adenocarcinoma negative for staining; E, F: Carcinoma tissues of the ampulla of Vater; E: Moderately differentiated tubular adenocarcinoma positive for staining. Note that LNγ2 is strongly positive in tumor cells at the invasive front; F: Well differentiated tubular adenocarcinoma negative for staining.

Figure 3 LNβ3 expression in biliary tract cancer tissues. A-C: Extrahepatic bile duct cancer tissues; A: Well differentiated tubular adenocarcinoma positive for invasive front dominant staining; B: Papillary adenocarcinoma positive for diffuse staining; C: Moderately differentiated tubular adenocarcinoma negative for staining; D-F: Gallbladder cancer tissues; A-C: Extrahepatic bile duct cancer tissues; D: Moderately differentiated tubular adenocarcinoma positive for invasive front dominant staining; E: Papillary adenocarcinoma positive for diffuse staining; G-I: Carcinoma tissues of the ampulla of Vater; G: Well differentiated tubular adenocarcinoma positive for invasive front dominant staining; H: Well differentiated tubular adenocarcinoma positive for diffuse staining; I: Well differentiated tubular adenocarcinoma negative for staining.

Figure 4 MMP7 expression in biliary tract cancer tissues. A, B: Extrahepatic bile duct cancer tissues; A: Moderately differentiated tubular adenocarcinoma positive for staining. Note that MMP7 is strongly positive in tumor cells at the invasive front; B: Papillary adenocarcinoma negative for staining; C, D: Gallbladder cancer tissues; C: Moderately differentiated tubular adenocarcinoma positive for staining; D: Well differentiated tubular adenocarcinoma negative for staining; E, F: Carcinoma tissues of the ampulla of Vater; E: Moderately differentiated tubular adenocarcinoma positive for staining. Note that MMP7 is strongly positive in tumor cells at the invasive front; F: Well differentiated tubular adenocarcinoma negative for staining.

Figure 5 Casein zymography of surgical specimen pairs of biliary tract carcinoma and adjacent nontumor tissue. N and T: Matched samples from nontumor and tumor tissue, respectively.

Figure 6 In vitro invasion assay with or without TIMP1 (250 ng/mL) in TFK-1 and siRNA transfectants. Each column indicates the means of three experiments; ^bP < 0.01.