Protective effect of tea polyphenols against paracetamol-induced hepatotoxicity in mice is significanly correlated with cytochrome P450 suppression

Figure 1 Western blot analysis of protein for CYP2E1 in liver tissue of mice showing representative bands of each group (A) and normalized densitometic ratios of CYP2E to β-actin (B). ^bP < 0.01 vs control group; ^dP < 0.01 vs model group.

Figure 2 Changes of HE staining in liver tissue of mice 24 h after paracetamol administration (× 100) in control group with a normal central lobular region (A), model group with some central lobular hepatocyte necrosis and microvesicular fatty change (B), low TP dose group (C), medium TP dose group (D) and high TP dose group (E). The pathological change of liver was much milder in different TP dose groups than in model group.

Figure 3 Immunohistochemical staining showing expression of CYP2E1 (A) and CYP1A2 (B) in liver tissue of mice 24 h after paracetamol administration (× 400) in control group (a), model group (b), low TP dose group (c), medium TP dose group (d) and high TP dose group (e). The brown or dark brown stained cells were considered positive.

Figure 4 RT-PCR analysis of mRNA for CYP2E1 and CYP1A2 expression in liver tissue of mice showing representative bands of each group (A) and normalized densitometic ratio of CYP2E and CYP1A2 to GAPDH (B). ^aP < 0.05 vs model group; ^bP < 0.01 vs control group; ^dP < 0.01 vs model group.