Effects of Wei Chang An on expression of multiple genes in human gastric cancer grafted onto nude mice

Figure 1 HPLC print of 120 mg/mL WCA. HPLC: High Performance Liquid Chromatography; WCA: Wei Chang An.

Figure 2 PCNA expression detected by IHC (A, B) and Apoptotic cell detected by TUNEL (C, D) in various groups of human gastric gastric adenocarcinoma cell line SGC-7901 grafted onto nude mouse. PCNA is predominantly localized at nuclei. A: NS Control group (IHC, × 400); B: WCA group (IHC, × 400); Apoptotic cell labeled in various groups; C: NS Control group (TUNEL, × 400); D: WCA group (TUNEL, × 400).

Figure 3 Cleaved Caspase-3 (Asp 175) expression in various groups of xenografts. Cleaved Caspase-3 is predominantly localized at cytoplasmic and perinuclear. A: NS Control group (IHC, × 400); B: WCA group (IHC, × 400).

Figure 4 Phospho-Tyr705-Stat3 and Bcl-2 expression in various groups of human ga-stric adenocarcinoma cell line SGC-7901 grafted onto nude mouse detected by IHC. P-Stat3 is predominantly localized at nuclei. Bcl-2 is predominantly localized at cytoplasmic and nuclear membrane. A: NS Control group: positive staining of P-Stat3 in xenograft cells (IHC, × 400); B: WCA group: positive staining of P-Stat3 in xenograft cells (IHC, × 400); C: NS Control group: positive staining of Bcl-2 in xenograft cells (IHC, × 400); D: WCA group: positive staining of Bcl-2 in xenograft cells (IHC, × 400).