Feeding behavior and gene expression of appetite-related neuropeptides in mice lacking for neuropeptide Y Y5 receptor subclass

Figure 1 Fasting (48 h)-induced change in food intake (A) and water intake (B) in NPY Y5-KO mice. Fasting (48 h)-induced change in daily amounts of food intake and water intake in Y5-KO mice was measured and compared with those in wild-type (C57BL/6N) mice at 10 wk old. ^aP < 0.05 (unpaired Student’s t test).

Figure 2 Growth curves of wild-type (C57BL/6N) and NPY Y5-KO mice from 4 to 69 wk old. Data are the mean ± SE. From 4 to 40 wk old, the body weight of Y5-KO mice was twice as much as that of wild-type mice.

Figure 3 Change in feeding and drinking in NPY Y5-KO mice. Daily amounts of food intake (A) and water intake (B) in Y5-KO mice were measured and compared with those in wild-type (C57BL/6N) mice at the same age (from 11 to 69 wk old). ^aP < 0.05, ^bP < 0.01 (unpaired Student’s t test). M: Male; F: Female.

Figure 4 Effect of fasting on appetite-related gene expression in hypothalamus. Fasting (48 h)-induced change in gene expression of appetite-related genes (A: NPY; B: AgRP; C: CART; D: POMC) in Y5-KO mice was measured in the hypothalamus and change in signal intensity was quantitated in panel E at 10 wk old. 3v: Third ventricle; NPY: neuropeptide Y; AgRP: agouti-related protein; MCH: melanin-concentrating hormone; CART: cocaine-amphetamine-regulated transcript; POMC: proopiomelanocortin.