Immunogenicity and immunoprotection of recombinant PEB1 in Campylobacter-jejuni-infected mice

Figure 1 Analysis of expression pattern of recombinant protein by SDS-PAGE. Lane 1: protein marker; lanes 2-3: E. coli BL (DE3) transformed with pET28a(+) and pET28a(+)-peb1A respectively after induced with IPTG for 4 h; lanes 4-5: supernatant and precipitate of sonicated broken E. coli BL (DE3) transformed with pET28a(+)-peb1A; lanes 6-7: Purified recombinant protein.

Figure 2 OD values of specific serum IgG antibody levels after immunization with rPEB1.

Figure 3 Stimulation index of immunized BALB/c mice spleen lymphocytes stimulated by intramuscular injection of rRPEB1.

Figure 4 Survival of BALB/c mice immunized with rPEB1 after oral challenge with wild-type C. jejuni in control and vaccination groups.