Immunohistochemical localization of glutathione S-transferase-pi in human colorectal polyps

Figure 1 Sections of human colorectal adenoma (HE, × 150). A: Low-grade dysplasia; B: Mild-grade dysplasia; C: High-grade dysplasia.

Figure 2 Transmission electron micrograph, routine double staining with uranyl acetate and lead citrate. Nucleus (N), nucleoli (n), mucus granules (m), microvilli (mv). A: From a low-grade dysplasia adenoma (× 5900), desmosomes (d); B: From a mild-grade dysplasia adenoma, desmosomes (arrows); C: From a mild-grade dysplasia tissue (× 11 700), free polyribosomes (arrows), basal lamina (bl); D: From a high-grade dysplasia adenoma (× 5900), intracellular junctions (arrows).

Figure 3 GST-pi positive cells (HE, × 100). A: In low-grade dysplasia colorectal adenoma; B: In mild-grade dysplasia colorectal adenoma, moderate immunoreactivity (a) and strong immunoreactivity (b); C: In high-grade dysplasia colorectal adenoma.

Figure 4 A: Electron micrograph from a mild-grade dysplasia adenoma showing GST-pi positive particles located in ribosomes and nucleus (arrows, × 42 000); B: Ultrathin section from a high-grade dysplasia adenoma showing GST-pi positive particles located in cytoplasmic membranes and nuclear membrane (with uranylacetate and lead citrate arrows, × 57 000).